Description
Senior Research Associate, Vector ( In-Vitro ) Biology
Affinia Therapeutics is an innovative gene therapy company focused on broadening the reach of gene therapy. Our proprietary Affinia Rationally-designed Therapies (ART) platform is created to address key limitations of conventional gene therapies, by developing novel capsids, novel promoters, and novel manufacturing approaches to developing gene therapies for rare and prevalent devastating diseases. We are backed by a strong syndicate of life science investors and have ambitious plans to have a dramatic impact on the lives of patients around the world.
As an early employee you will play a key role in setting our culture and values. You will be working alongside the founding management team who have a track record of success in the industry. The pace of work is dynamic, fast and fun. We offer competitive salary and benefits with the ability to make a difference.
Position Summary
We are seeking a creative intellectually curious, and thorough individual to join the In Vitro Biology Team focused on a variety of Therapeutic Programs in the Vector Science group. This is a fast-paced, lab-focused role centering on the creation and in vitro testing of viral vector therapeutic candidates. In this role, you will have the opportunity to learn new skills and present your research. This position reports to the Vector Science Therapeutic Lead.
Primary Responsibilities
- Create and execute cloning strategies to generate AAV genome constructs for viral therapeutic candidates
- Perform cell culture: maintain and bank cell lines, and execute cell-based experiments such as transfections, transductions, and cell titer assays
- Quantitate DNA and RNA using ddPCR, qPCR, and RT-QPCR techniques
- Develop, validate, and utilize protein expression assays, such as ELISA and Western Blot
- Write, prepare and present technical data, technical reports and standard operating procedures (SOPs) for internal use and tech transfer.
- Maintain an up-to-date notebook, participate in meetings, prepare presentations, and communicate to leadership as needed.
Minimum Qualifications
- Bachelor's Degree in molecular or cell biology with three years relevant experience.
- Hands-on experience with basic molecular biology techniques including e.g. Western blots, ELISA, plasmid production, ddPCR and qPCR highly desired
- Stem cell culture and AAV experience a plus
- Ability to thrive in a fast paced environment flexibly and collaboratively
- Operate daily with Integrity, Teamwork and Passion
EEO Statement: Affinia Therapeutics is proud to be an equal opportunity workplace. We are committed to equal employment opportunity regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity or Veteran status. If you have a disability or special need that requires accommodation, please let us know.
