Endotoxin Testing: Resolving Interference and Test Validation Training Course :

Common causes of interference with the bacterial endotoxins test are presented in this course and specific methods to eliminate them are given. Strategies for detecting interference; validation of special treatments to overcome interference and validation of the test for endotoxins by performing the Pharmacopeial Test for Interfering Factors are all addressed. Inclusion of these elements in an LAL testing program will result in compliant testing.<br><br> This 90-minute course will benefit both the experienced manager who has been supervising endotoxin testing and the novice technician who wishes to understand and overcome interference with the test.<br><br> <b>Module 1: Causes of Interference with the <i>Limulus</i> Amebocyte Lysate (LAL) Test for Endotoxin</b><li> Introduction</li> <li>Common causes for interference and their elimination<br> &ndash; pH; Salts; Chelating Agents; Proteins (including blood serum and plasma); Agents that Denature Proteins; Agents that Affect Endotoxin; Lipids and Liposomes</li><br><br> <b>Module 2: Overcoming Interference in Endotoxin Testing</b><li> Testing for Interference</li> <li>Strategies to Overcome Inhibition<br> &ndash; Dilution; Use of Buffer; Sample Treatments including: use of other test methods, chemical modification and ultrafiltration</li><br><br> <b>Module 3: Validation of Sample Treatments and the Pharmacopeial Test for Interfering Factors for Validation of the Endotoxins Test</b><li> The Test for Interfering Factors<br> &ndash; Gel-clot Method<br> &ndash; Photometric Methods<br></li> <li>Conclusion</li>